Comparison of microarray mRNA profiles expressed in longissimus dorsi in porcine of different ages

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Title:	Comparison of microarray mRNA profiles expressed in longissimus dorsi in porcine of different ages
Authors:	張慧玫 Chang, Whei-meih
Contributors:	生物資訊學系 Bioinformatics
Keywords:	生長期mRNA表現;cDNA晶片;豬背長肌生長 mRNA growth performance;cDNA microarray;porcine longissimus
Date:	2010
Issue Date:	2014-06-27 01:05:51 (UTC+8)
Abstract:	摘要本研究利用生物晶片以Cy3/Cy5的訊號比判定對於豬肉背長肌中發育有影響的基因，本研究嘗試利用六頭不同月齡在目前最常被飼養的雜種豬來尋找影響豬背最長肌生長的基因。將此六頭豬以年齡區分，可分成三個時期：哺乳期(一週)、成長期(三個月)、成豬(八個月)，取出total RNA樣本之後將帶有人類cDNA序列探針的商用Agilent生物晶片偵測其中的基因轉錄的表現量，藉此尋找基因的變化，經過校正、排除極端值、矛盾點之後，將log2為底的Cy3/Cy5(M值)大於1或小於-1進行倍數篩選，經上述步驟之後，本研 Abstract We use mRNA microarrays to study possible differential expressed genes responsible for growth of porcine longissimus dorsi muscle. Six sibling pigs of the most commonly reared hybrid species in Taiwan were collected at three stages: the birth per
Appears in Collections:	[Department of Bioinformatics] Journal Articles

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